|Circuit part list|
|300 0101||Silicone tubing, Luer fitting on each side||i.d = 1 mm; l = 1 m|
|300 0102||Silicone tubing, Luer fitting on each side||i.d = 3 mm; l = 1 m|
|300 0210||Luer tubing adapter, male and female, 5 each||i.d. = 1.6 mm|
|300 0220||Luer tubing adapter, male and female, 5 each||i.d. = 3.2 mm|
|300 0303||Gas-permeable media bag||V = 1000 ml|
|300 0400||Sterile Luer coupling, pack of 10|
|300 0511||Pump ISMATEC IPC-4||0.002 ... 44 ml/min|
|300 0512||Pump ISMATEC IPC-N4||0.0004 ... 11 ml/min|
|300 0513||Pump ISMATEC IPC-8||0.002 ... 44 ml/min|
|300 0514||Pump ISMATEC IPC-N8||0.0004 ... 11 ml/min|
|300 0521||Pump tubing, with 2 stoppers, Luer fitting on each side||i.d. = 1 mm|
|300 0522||Pump tubing, with 2 stoppers, Luer fitting on each side||i.d. = 3 mm|
|300 0710||Y-connector, pack of 2||i.d. = 1.6 mm|
|300 0720||Y-connector, pack of 2||i.d. = 3.2 mm|
Perfusion circuit PCS3c
In the case of different culture media flowing through the upper and lower chambers, a multi-channel pump is used to generate similar flow rates in both circuits. Alternatively, the flow from a single outlet from the media reservoir may be split by using Y-connectors. For convenience, both circuits may be merged after leaving the chamber using a Y-connector.
Perfusion circuit TCS2c
Different flow rates in the inner and outer circuits can be generated using different diameters of tubing. Using sterile couplings mounted between the Luer connectors, the media bag can be changed without interrupting the media flow.
Perfusion circuit FCS1c
In this open circuit, the culture medium passes through the flow chamber at a shear rate defined by the pump and is collected in another reservoir. The culture medium is pre-conditioned in a gas-permeable media bag by equilibrating in a CO2 incubator. Using impermeable tubing, the partial gas pressure remains stable until the medium reaches the cell monolayer.