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perfusion culture systems

flow chamber system FCS1c

The bottom of the flow channel is formed by the corpus, the side wall – by the gasket, and its top – by the cover slip. The cover slip may be pre-coated with a cell monolayer in a multi-well plate, and will be fixated on the flow chamber by a clip-on spring jacket. This allows an easy exchange of the cultures to be investigated. Modifying the thickness of the gasket and/or the width of its gap, the channel geometry may be altered with the aim to use a whole range of well-defined shear stress. The flow chamber was designed to allow fluorescence microscopic observation of a perifused cell layer at high resolution.

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Flow chamber FCS1c
OFFER NO.PRODUCTSPECIFICATION
303 0000Flow chamber, complete
303 0100Main corpus with tubing adapter
303 0510Clip-on spring, pack of 10
303 0521Cover slips, pack of 10, thermanoxø 25 mm
303 0522Cover slips, pack of 10, glassø 25 mm
303 0530Chamber tubing, complete with Luer fitting, pack of 2
303 0541Silicon gasket, pack of 10h = 0.3 mm
303 0543Silicon gasket, pack of 10h = 0.5 mm

Specific advantages

  • Ability to observe cultures by fluorescence microscopy
  • Use of coverslips pre-coated with cell cultures
  • Easy assembling by means of a clip-on spring jacket
  • Generation of defined shear stresses in the flow channel
  • Possibility to position the monolayer at the bottom or on top of the media flow
  • Variety in flow channel geometry by modifying the gasket
  • Leak-proof set-up of the flow chamber

Technical data

Channel length: 15 mm
Channel width : ≥ 2 mm
Channel height: 300 µm
Working distance: 1 mm
Shear stress: 0 - 3 Pa

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Copyright: 2020 provitro AG,
Charitéplatz 1, 10117 Berlin

Contact provitro

provitro AG
Charitéplatz 1, 10117 Berlin
Tel: +49.30.450 578 358
Email: sales@provitro.com

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immunohistochemistry
in situ hybridisation services
tissue microarrays (TMA)
iConTMA®
digital pathology
primary cell cultures
culture media
perfusion culture systems
cytokines
ScienCell products
order information
imprint
data Protection
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provitro

Provitro stands for more than ten years of experience in commercialisation of cell culture technologies and tissue microarrays backed up by scientific knowledge and resources of immunohistochemical analyses at the Pathological Institute of Charité Universitätsmedizin Berlin. We have added medical research to our own know-how in biotechnology to create solutions to common problems in life sciences. Provitro’s team has raised its sights to the interface between research and development to hammer out brand-new solutions to the benefit of future research projects. These are the hallmarks: competence, vision and sustainability.