header image with person and cell structuresheader image with person and cell structuresheader image with person and cell structuresheader image with person and cell structures

perfusion culture systems

perfusion culture system PCS3c

Both bottom and top of the leak-proof perfusion chamber have integrated glass windows to allow not only a visual control of the culture, but also its microscopic observation. To disconnect the chamber temporarily from the system, a coupling that maintains sterility may be used. The universal lower chamber unit has an build-in air bubble trap avoiding malnutrition of parts of the culture. By designing two different upper chamber units, it guarantees a high variability of applicable cell and tissue supports (membranes, filters, well inserts, etc.). The system may be completed with a cooling plate that allows a bench-top storage of the media bag at low temperature.

download Detail view

Perfusion chamber PCS3c
301 0100Perfusion chamber, lower universal unit
301 0200Perfusion chamber, upper membrane-housing unit
301 0300Perfusion chamber, upper well insert-housing unit
301 0411Membrane, Millicell, pack of 10ø Pores 0.4 µm
301 0422Well-insert, Millicell PTFE, pack of 5ø Pores 0.4 µm, transparent
301 0501O-ring-set
301 0520Chamber tubing (complete with Luer fitting and O-ring), pack of 4
301 0503Inspection windows, pack of 2

Specific advantages

  • Ability to observe cultures microscopically inside the perfusion chamber
  • Variety of cell and tissue supports applicable (membranes, filters, well inserts)
  • Built-in air bubble trap that avoids poor nutrition or partial drying of the culture
  • Two-compartment chamber allows the application of a medium gradient, e.g. for drug testing
  • Leak-proof set-up of the perfusion chamber
  • Use of media bags for easy handling

Technical data

Filling volume of lower unit: 1.5 ml
Filling volume of upper unit: 6.5 ml
Height of assembled chamber: 28.0 mm
Diameter of chamber: 53.0 mm
Height of lower unit: 10.0 mm
Height of upper unit: 18.0 mm
Diameter of membrane: 25.0 mm
Diameter of well insert: 27.0 mm
Height of well insert: 12.0 mm
Recommended flow rate: 1 - 10 ml/h